rabbit anti rat antibodies against nrf2  (Proteintech)

Journal: World Journal of Diabetes

Article Title: Effect of fish scale ointment on diabetic foot ulcer by inducing ferroptosis via the nuclear factor E2-related factor 2 pathway

doi: 10.4239/wjd.v16.i12.111789

Figure Lengend Snippet: Levels of ferroptosis-related proteins in rat wound tissues. A: Western blot bar plot of the proteins; B: Nuclear factor E2-related factor 2 protein expression level; C: Heme oxygenase-1 protein expression level; D: Glutathione peroxidase 4 protein expression level. a P < 0.05 vs control; b P < 0.05 vs model; c P < 0.05 vs fish scale collagen. GPX4: Glutathione peroxidase 4; HO-1: Heme oxygenase-1; Nrf2: Nuclear factor E2-related factor 2; FSC: Fish scale collagen; Lip-1: Liproxstatin-1.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-rat primary antibodies against Nrf2 (No. 12721, 1:1000), heme oxygenase-1 (HO-1) (No. 70081, 1:1000), glutathione peroxidase 4 (GPX4; No. 52455, 1:1000), and β-actin (No. 4967, 1:1000), all from Cell Signaling Technology, United States.

Techniques: Western Blot, Expressing, Control

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: Preparation of RCDs/UA@Lipo-HAMA and schematic diagram of therapeutic mechanisms against adhesion. ( A ) Preparation of RCDs/UA@Lipo for dual-drug co-loading. ( B ) The mixture of RCDs/UA@Lipo and HAMA was precisely applied to the periphery of the sutured tendon model and photocured using 365 nm UV irradiation. This process solidified the HAMA, enabling a localized and sustained release of RCDs and UA. ( C ) RCDs/UA@Lipo-HAMA diminishes oxidative stress by activating Nrf2/HO-1, reducing CD68, and iNOS, and suppressing fibrosis proteins, thereby lessening inflammation and fibrosis, reducing tendon adhesion, and promoting healing.

Article Snippet: After permeabilized and blocked, the sections were incubated with rabbit anti-rat antibodies against Nrf2 (1:200; Cat# 80593-1-RR, Proteintech, USA), HO-1 (1:200; Cat#10701-1-AP, Proteintech, USA), CD68 (1:300; GB11067, Servicebio, China), and iNOS (1:200; Cat#18985-1-AP, Proteintech, USA) at 4°C overnight.

Techniques: Irradiation